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РАЗРАБОТКА ТЕХНОЛОГИИ ОЧИСТКИ РЕКОМБИНАНТНОГО СЛИТОГО ПОЛИПЕПТИДА ИЗ КАПСИДНЫХ БЕЛКОВ ВИРУСА ДЕНГЕ

https://doi.org/10.20914/2304-4691-2025-2-30-32

About the Authors

В. Лемешева
ФГБУ СПбНИИВС ФМБА России
Russian Federation


Д. Поляков
ФГБУ СПбНИИВС ФМБА России
Russian Federation


Н. Савельев
ФГБУ СПбНИИВС ФМБА России
Russian Federation


Ю. Плетюхина
ФГБУ СПбНИИВС ФМБА России
Russian Federation


С. Рабдано
ФГБУ СПбНИИВС ФМБА России
Russian Federation


References

1. Byk L.A., Gamarnik A.V. Properties and functions of the dengue virus capsid protein // Annual Review of Virology. 2016. Vol. 3. № 1. P. 263-281.

2. Jonsson C.B., Gallegos J., Fero P. et al. Purification and characterization of the Sin Nombre virus nucleocapsid protein expressed in Escherichia coli // Protein Expression and Purification. 2001. Vol. 23. № 1. P. 134-141.

3. Gil L., Cobas K., Lazo L. et al. A tetravalent formulation based on recombinant nucleocapsid-like particles from dengue viruses induces a functional immune response in mice and monkeys // Journal of Immunology. 2016. Vol. 197. № 9. P. 3597-3606.

4. Brudenell E.L., Pohare M.B., Zafred D. et al. Efficient overexpression and purification of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteins in Escherichia coli // Biochemical Journal. 2024. Vol. 481. № 11. P. 669-682.

5. Chen C., Zhang Z., Zheng Q. et al. Purification and inhibitor screening of the full-length SARS-CoV-2 nucleocapsid protein // Journal of Immunology. 2025. Vol. 202. № 1. P. 1-10. doi: 10.4049/jimmunol.1600927


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