CRISPR/CAS9 КАК АЛЬТЕРНАТИВА КОНТРСЕЛЕКЦИИ ПО URA3 ДЛЯ ПРИЦЕЛЬНОЙ ИНТЕГРАЦИИ ДНК В ГЕНОМ SACCHAROMYCES CEREVISIAE

1. Boeke JD, Trueheart J, Natsoulis G, Fink GR. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 1987;154:164-175.

2. Vyas VK, Bushkin GG, Bernstein DA, et al. New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi. mSphere. 2018;3(2):e00154-18.

3. Mannhaupt G, Schnall R, Karpov V, Vetter I, Feldmann H. Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast. FEBS Lett. 1999;450(1-2):27-34.

4. González-Ramos D, van den Broek M, van Maris AJ, Pronk JT, Daran JM. Genome-scaleanalyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation. Biotechnol Biofuels. 2013;6(1):48.

5. Karpov DS, Spasskaya DS, Nadolinskaia NI, Tutyaeva VV, Lysov YP, Karpov VL. Deregulation of the 19S proteasome complex increases yeast resistance to 4-NQO and oxidative stress via upregulation of Rpn4 -and proteasome-dependent stress responsive genes. FEMS Yeast Res. 2019;19(2):10.1093/femsyr/foz002.