actbiotechАктуальная биотехнологияTopical biotechnology2304-4691ВГУИТ10.20914/2304-4691-2020-3-308actbiotech-4867Research ArticleСтатьиВЛИЯНИЕ ГИДРОФИЛЬНОСТИ АРОМАТИЧЕСКИХ УГЛЕВОДОРОДНЫХ ЗАМЕСТИТЕЛЕЙ ПО С5 ПОЛОЖЕНИЮ ПИРИМИДИНОВОГО ЦИКЛА ДЕЗОКСИУРИДИНТРИФОСФАТОВ НА ЭФФЕКТИВНОСТЬ ПЦР-АМПЛИФИКАЦИИ ДНК ПОЛИМЕРАЗАМИИЗаседателеваО. А.noemail@neicon.ruСуржиковС. А.noemail@neicon.ruШершовВ. Е.noemail@neicon.ruМифтаховР. А.noemail@neicon.ruЮрасовД. А.noemail@neicon.ruКузнецоваВ. Е.noemail@neicon.ruЧудиновА. В.noemail@neicon.ruИнститут молекулярной биологии им. В.А. Энгельгардта РАНRussian Federation20202609202003308308Copyright © Заседателева О.А., Суржиков С.А., Шершов В.Е., Мифтахов Р.А., Юрасов Д.А., Кузнецова В.Е., Чудинов А.В., 20202020Заседателева О.А., Суржиков С.А., Шершов В.Е., Мифтахов Р.А., Юрасов Д.А., Кузнецова В.Е., Чудинов А.В.Заседателева О.А., Суржиков С.А., Шершов В.Е., Мифтахов Р.А., Юрасов Д.А., Кузнецова В.Е., Чудинов А.В.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actbio-vsuet.ru/jour/article/view/4867ReferencesJäger S., Rasched G., Kornreich-Leshem H., Engeser M., Thum O., Famulok M. A versatile toolbox for variable DNA functionalization at high density. J Am Chem Soc. 2005. 127, 15071-15082.Jäger S., Rasched G., Kornreich-Leshem H., Engeser M., Thum O., Famulok M. A versatile toolbox for variable DNA functionalization at high density. J Am Chem Soc. 2005. 127, 15071-15082.Kuwahara M., Nagashima J., Hasegawa M., Tamura T., Kitagata R., Hanawa K., Hososhima S., Kasamatsu T., Ozaki H., Sawai H. Systematic characterization of 2' -deoxynucleoside-5' -triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA. Nucleic Acids Res. 2006. 34, 5383- 5394.Kuwahara M., Nagashima J., Hasegawa M., Tamura T., Kitagata R., Hanawa K., Hososhima S., Kasamatsu T., Ozaki H., Sawai H. Systematic characterization of 2' -deoxynucleoside-5' -triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA. Nucleic Acids Res. 2006. 34, 5383- 5394.Zasedateleva O.A., Surzhikov S.A., Shershov V.E., Miftakhov R.A., Yurasov D.A., Kuznetsova V.E., Chudinov A.V.PCR incorporation of dUMPs modified with aromatic hydrocarbon substituents of different hydrophilicities: Synthesis of C5-modified dUTPs and PCR studies using Taq, Tth, Vent (exo-) and Deep Vent (exo-) polymerases. Bioorg Chem. 2020. 99:103829.Zasedateleva O.A., Surzhikov S.A., Shershov V.E., Miftakhov R.A., Yurasov D.A., Kuznetsova V.E., Chudinov A.V.PCR incorporation of dUMPs modified with aromatic hydrocarbon substituents of different hydrophilicities: Synthesis of C5-modified dUTPs and PCR studies using Taq, Tth, Vent (exo-) and Deep Vent (exo-) polymerases. Bioorg Chem. 2020. 99:103829.Zasedateleva O.A., Vasiliskov V.A., Surzhikov S.A., Kuznetsova V.E., Shershov V.E., Guseinov T.O., Smirnov I.P., Yurasov R.A., Spitsyn M.A., Chudinov A.V. dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. Nucleic Acids Res. 2018. 46, e73.Zasedateleva O.A., Vasiliskov V.A., Surzhikov S.A., Kuznetsova V.E., Shershov V.E., Guseinov T.O., Smirnov I.P., Yurasov R.A., Spitsyn M.A., Chudinov A.V. dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. Nucleic Acids Res. 2018. 46, e73.
The authors declare that there are no conflicts of interest present.