actbiotechАктуальная биотехнологияTopical biotechnology2304-4691ВГУИТ10.20914/2304-4691-2019-3-625-627actbiotech-4389Research ArticleСтатьиКОНСТРУИРОВАНИЕ И ПРИМЕНЕНИЕ ВЫРОЖДЕННЫХ ПРАЙМЕРОВГоринаС. С.noemail@neicon.ruТопорковаЯ. Ю.noemail@neicon.ruКазанский научный центр РАНRussian Federation20191809201903625627Copyright © Горина С.С., Топоркова Я.Ю., 20192019Горина С.С., Топоркова Я.Ю.Горина С.С., Топоркова Я.Ю.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actbio-vsuet.ru/jour/article/view/4389ReferencesLilit Garibyan and Nidhi Avashia. Research Techniques Made Simple: Polymerase Chain Reaction (PCR). J Invest Dermatol. 2013. 133 (3). P.1-4.Lilit Garibyan and Nidhi Avashia. Research Techniques Made Simple: Polymerase Chain Reaction (PCR). J Invest Dermatol. 2013. 133 (3). P.1-4.Compton T (1990). Degenerate primers for DNA amplification. pp. 39-45 in: PCR Protocols (Innis, Gelfand, Sninsky and White, eds.);Compton T (1990). Degenerate primers for DNA amplification. pp. 39-45 in: PCR Protocols (Innis, Gelfand, Sninsky and White, eds.);Brash AR. Mechanistic aspects of СYР74 allene oxide synthases and related cytochrome Р450 enzymes. Phytochemistry. 2009 Sep; 70(13-14) : 1522-31. doi: 10.1016/j.phytochem.2009.08.005. Epub 2009 Sep 9. Review.Brash AR. Mechanistic aspects of СYР74 allene oxide synthases and related cytochrome Р450 enzymes. Phytochemistry. 2009 Sep; 70(13-14) : 1522-31. doi: 10.1016/j.phytochem.2009.08.005. Epub 2009 Sep 9. Review.Toporkova YY, Gorina SS, Bessolitsyna EK, Smirnova EO, Fatykhova VS, Brühlmann F, Ilyina TM, Mukhtarova LS, Grechkin AN. Double function hydroperoxide lyases/epoxyalcohol synthases (СYР74С) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr; 1863(4) : 369-378. doi: 10.1016/j.bbalip.2018.01.002. Epub 2018 Jan 8.Toporkova YY, Gorina SS, Bessolitsyna EK, Smirnova EO, Fatykhova VS, Brühlmann F, Ilyina TM, Mukhtarova LS, Grechkin AN. Double function hydroperoxide lyases/epoxyalcohol synthases (СYР74С) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Apr; 1863(4) : 369-378. doi: 10.1016/j.bbalip.2018.01.002. Epub 2018 Jan 8.Bak S, Beisson F, Bishop G, Hamberger B, Höfer R, Paquette S, Werck-Reichhart D. Cytochromes Р450. Arabidopsis Book. 2011; 9:е0144. doi: 10.1199/tab.0144. Epub 2011 Oct 6.Bak S, Beisson F, Bishop G, Hamberger B, Höfer R, Paquette S, Werck-Reichhart D. Cytochromes Р450. Arabidopsis Book. 2011; 9:е0144. doi: 10.1199/tab.0144. Epub 2011 Oct 6.Gogolev YV, Gorina SS, Gogoleva NE, Toporkova YY, Chechetkin IR, Grechkin AN. Green leaf divinyl ether synthase: gene detection, molecular cloning and identification of a unique СYР74В subfamily member. Biochim Biophys Acta. 2012 Feb; 1821(2) : 287-94. doi: 10.1016/j.bbalip.2011.11.003. Epub 2011 Nov 23.Gogolev YV, Gorina SS, Gogoleva NE, Toporkova YY, Chechetkin IR, Grechkin AN. Green leaf divinyl ether synthase: gene detection, molecular cloning and identification of a unique СYР74В subfamily member. Biochim Biophys Acta. 2012 Feb; 1821(2) : 287-94. doi: 10.1016/j.bbalip.2011.11.003. Epub 2011 Nov 23.Gorina SS, Toporkova YY, Mukhtarova LS, Chechetkin IR, Khairutdinov BI, Gogolev YV, Grechkin AN. Detection and molecular cloning of СYР74Q1 gene: identification of Ranunculus acris leaf divinyl ether synthase. Biochim Biophys Acta. 2014 Sep; 1841(9) : 1227-33. doi: 10.1016/j.bbalip.2014.05.005. Epub 2014 May 24.Gorina SS, Toporkova YY, Mukhtarova LS, Chechetkin IR, Khairutdinov BI, Gogolev YV, Grechkin AN. Detection and molecular cloning of СYР74Q1 gene: identification of Ranunculus acris leaf divinyl ether synthase. Biochim Biophys Acta. 2014 Sep; 1841(9) : 1227-33. doi: 10.1016/j.bbalip.2014.05.005. Epub 2014 May 24.Toporkova YY, Fatykhova VS, Gorina SS, Mukhtarova LS, Grechkin AN. Epoxyalcohol Synthase RjЕАS (СYР74А88) from the Japanese Buttercup (Ranunculus japonicus): Cloning and Characterization of Catalytic Properties. Biochemistry (Mosc). 2019 Feb; 84(2) : 171-180. doi: 10.1134/S0006297919020081.Toporkova YY, Fatykhova VS, Gorina SS, Mukhtarova LS, Grechkin AN. Epoxyalcohol Synthase RjЕАS (СYР74А88) from the Japanese Buttercup (Ranunculus japonicus): Cloning and Characterization of Catalytic Properties. Biochemistry (Mosc). 2019 Feb; 84(2) : 171-180. doi: 10.1134/S0006297919020081.

The authors declare that there are no conflicts of interest present.