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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">actbiotech</journal-id><journal-title-group><journal-title xml:lang="ru">Актуальная биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Topical biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2304-4691</issn><publisher><publisher-name>ВГУИТ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2304-4691-2021-1-261-264</article-id><article-id custom-type="elpub" pub-id-type="custom">actbiotech-3806</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>ЭКСПЕРИМЕНТАЛЬНОЕ МОДЕЛИРОВАНИЕ ПЛОСКОКЛЕТОЧНОГО РАКА ГОЛОВЫ И ШЕИ С ИСПОЛЬЗОВАНИЕМ БИОПОЛИМЕРНЫХ КОНСТРУКЦИЙ ИЗ ПОЛИ 3 ОКСИБУТИРАТА</article-title><trans-title-group xml:lang="en"><trans-title></trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Холина</surname><given-names>М. С.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Белишев</surname><given-names>Н. В.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бонарцев</surname><given-names>А. П.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Святославов</surname><given-names>Д. С.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Самойлова</surname><given-names>С. И.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Решетов</surname><given-names>И. В.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шайтан</surname><given-names>К. В.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>Московский государственный университет им. М.В. Ломоносова</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-2"><institution>Первый МГМУ им. И.М. Сеченова Минздрава России</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>22</day><month>09</month><year>2021</year></pub-date><volume>0</volume><issue>1</issue><fpage>261</fpage><lpage>264</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Холина М.С., Белишев Н.В., Бонарцев А.П., Святославов Д.С., Самойлова С.И., Решетов И.В., Шайтан К.В., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Холина М.С., Белишев Н.В., Бонарцев А.П., Святославов Д.С., Самойлова С.И., Решетов И.В., Шайтан К.В.</copyright-holder><copyright-holder xml:lang="en">Холина М.С., Белишев Н.В., Бонарцев А.П., Святославов Д.С., Самойлова С.И., Решетов И.В., Шайтан К.В.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.actbio-vsuet.ru/jour/article/view/3806">https://www.actbio-vsuet.ru/jour/article/view/3806</self-uri></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">WHO report on cancer: setting priorities, investing wisely and providing care for all. Geneva: World Health Organization; 2020. Licence: CC BY-NC-SA 3.0 IGO.</mixed-citation><mixed-citation xml:lang="en">WHO report on cancer: setting priorities, investing wisely and providing care for all. Geneva: World Health Organization; 2020. Licence: CC BY-NC-SA 3.0 IGO.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Solomon B, Young RJ, Rischin D. Head and neck squamous cell carcinoma: Genomics and emerging biomarkers for immunomodulatory cancer treatments. Semin Cancer Biol. 2018 Oct; 52 (Pt 2):228-240. doi: 10.1016/j.semcancer.2018.01.008. Epub 2018 Jan 31. PMID: 29355614.</mixed-citation><mixed-citation xml:lang="en">Solomon B, Young RJ, Rischin D. Head and neck squamous cell carcinoma: Genomics and emerging biomarkers for immunomodulatory cancer treatments. Semin Cancer Biol. 2018 Oct; 52 (Pt 2):228-240. doi: 10.1016/j.semcancer.2018.01.008. Epub 2018 Jan 31. PMID: 29355614.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Colella G, Fazioli F, Gallo M, De Chiara A, Apice G, Ruosi C, Cimmino A, de Nigris F. Sarcoma Spheroids and Organoids-Promising Tools in the Era of Personalized Medicine. Int J Mol Sci. 2018 Feb 21; 19(2):615. doi: 10.3390/ijms19020615. PMID: 29466296; PMCID: PMC5855837.</mixed-citation><mixed-citation xml:lang="en">Colella G, Fazioli F, Gallo M, De Chiara A, Apice G, Ruosi C, Cimmino A, de Nigris F. Sarcoma Spheroids and Organoids-Promising Tools in the Era of Personalized Medicine. Int J Mol Sci. 2018 Feb 21; 19(2):615. doi: 10.3390/ijms19020615. PMID: 29466296; PMCID: PMC5855837.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Меньших К.А., Воинова В.В., Бонарцев А.П. Создание 3D клеточных сфероидов на микросферах из поли 3 оксибутирата. «Биотехнология: наука и практика», VII Международная научно-практическая конференция, Севастополь, Россия, 16-20 сентября 2019.</mixed-citation><mixed-citation xml:lang="en">Меньших К.А., Воинова В.В., Бонарцев А.П. Создание 3D клеточных сфероидов на микросферах из поли 3 оксибутирата. «Биотехнология: наука и практика», VII Международная научно-практическая конференция, Севастополь, Россия, 16-20 сентября 2019.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">И.В. Демьянова, Е.А. Акулина, Т.Х. Труонг и др. Рост мезенхимальных стволовых клеток на пленках поли 3 оксибутирата, загруженных левофлоксацином и симвастатином / // Биологический журнал. - 2020. - № 4(15). - С. 4-9. - DOI 10.32743/2658-6460.2020.4.15.283.</mixed-citation><mixed-citation xml:lang="en">И.В. Демьянова, Е.А. Акулина, Т.Х. Труонг и др. Рост мезенхимальных стволовых клеток на пленках поли 3 оксибутирата, загруженных левофлоксацином и симвастатином / // Биологический журнал. - 2020. - № 4(15). - С. 4-9. - DOI 10.32743/2658-6460.2020.4.15.283.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">V.A., Akoulina E.A., Ivanova E.V., Kuznetsova E.S., Shaitan K.V., Bonartseva G.A. Biosynthesis of poly(3 - hydroxybutyrate) copolymers by Azotobacter chroococcum 7B: a precursor feeding strategy // Preparative Biochemistry and Biotechnology. - 2017. V. - 47. N. - 2. - P. 173-184.</mixed-citation><mixed-citation xml:lang="en">V.A., Akoulina E.A., Ivanova E.V., Kuznetsova E.S., Shaitan K.V., Bonartseva G.A. Biosynthesis of poly(3 - hydroxybutyrate) copolymers by Azotobacter chroococcum 7B: a precursor feeding strategy // Preparative Biochemistry and Biotechnology. - 2017. V. - 47. N. - 2. - P. 173-184.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Bartosh TJ, Ylöstalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, Lee RH, Choi H, Prockop DJ. Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci USA. 2010 Aug 3; 107(31):13724-9. doi: 10.1073/pnas.1008117107. Epub 2010 Jul 19. PMID: 20643923; PMCID: PMC2922230.</mixed-citation><mixed-citation xml:lang="en">Bartosh TJ, Ylöstalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, Lee RH, Choi H, Prockop DJ. Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci USA. 2010 Aug 3; 107(31):13724-9. doi: 10.1073/pnas.1008117107. Epub 2010 Jul 19. PMID: 20643923; PMCID: PMC2922230.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Krishnamurthy S, Nör JE. Orosphere assay: a method for propagation of head and neck cancer stem cells. Head Neck. 2013 Jul; 35(7):1015-21. doi: 10.1002/hed.23076. Epub 2012 Jul 13. PMID: 22791367; PMCID: PMC3887391.</mixed-citation><mixed-citation xml:lang="en">Krishnamurthy S, Nör JE. Orosphere assay: a method for propagation of head and neck cancer stem cells. Head Neck. 2013 Jul; 35(7):1015-21. doi: 10.1002/hed.23076. Epub 2012 Jul 13. PMID: 22791367; PMCID: PMC3887391.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">https://www.abcam.com/protocols/flow-cytometry-intracellular-staining-protocol</mixed-citation><mixed-citation xml:lang="en">https://www.abcam.com/protocols/flow-cytometry-intracellular-staining-protocol</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Kholina M.S., Menshikh K.A., Voinova V.V., Denisova K.R., Makhina T.K., Bonartseva G.A., Svyatoslavov D.S., Samoylova S.I., Reshetov I.V., Shaitan 1 K.V., Bonartsev A.P. The co-cultivation of human cancer HEp 2 with rat mesenchymal stem cells: low attachment vs. hanging drop spheroid culturing techniques. ESAO/TERMIS ONLINE Winter School in Jaca, Spain February 24-26, 2021, Online Conference, Испания, 24-26 февраля 2021.</mixed-citation><mixed-citation xml:lang="en">Kholina M.S., Menshikh K.A., Voinova V.V., Denisova K.R., Makhina T.K., Bonartseva G.A., Svyatoslavov D.S., Samoylova S.I., Reshetov I.V., Shaitan 1 K.V., Bonartsev A.P. The co-cultivation of human cancer HEp 2 with rat mesenchymal stem cells: low attachment vs. hanging drop spheroid culturing techniques. ESAO/TERMIS ONLINE Winter School in Jaca, Spain February 24-26, 2021, Online Conference, Испания, 24-26 февраля 2021.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
